600-401-420

antibody from Rockland Immunochemicals, Inc.

Targeting: PPARD FAAR, NR1C2, NUC1, NUCII

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-420

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-420, RRID:AB_2165928

Product name

Anti-PPAR delta (N terminal specific) (RABBIT) Antibody - 600-401-420

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1.

Matsushita N, Hassanein MT, Martinez-Clemente M, Lazaro R, French SW, Xie W, Lai K, Karin M, Tsukamoto H
PloS one 2017;12(8):e0181052

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Affinity Purified Anti-PPAR delta [Rabbit] is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates.  Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown).  Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.  Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4° C followed reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) (611-132-122) for 45 min at room temperature (800 nm channel, green).  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot