- PA5-29678 - Invitrogen Antibodies PPARD antibody

Submitted references Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Koh JH, Pataky MW, Dasari S, Klaus KA, Vuckovic I, Ruegsegger GN, Kumar AP, Robinson MM, Nair KS
Nature communications 2022 Apr 28;13(1):2324

Mitochondrial dysfunction in skeletal muscle contributes to the development of acute insulin resistance in mice.

Lee H, Ha TY, Jung CH, Nirmala FS, Park SY, Huh YH, Ahn J
Journal of cachexia, sarcopenia and muscle 2021 Dec;12(6):1925-1939

Mitochondrial Utilization of Competing Fuels Is Altered in Insulin Resistant Skeletal Muscle of Non-obese Rats (Goto-Kakizaki).

Lai N, Fealy CE, Kummitha CM, Cabras S, Kirwan JP, Hoppel CL
Frontiers in physiology 2020;11:677

PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise.

Koh JH, Hancock CR, Terada S, Higashida K, Holloszy JO, Han DH
Cell metabolism 2017 May 2;25(5):1176-1185.e5

Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells.

Rutz S, Kayagaki N, Phung QT, Eidenschenk C, Noubade R, Wang X, Lesch J, Lu R, Newton K, Huang OW, Cochran AG, Vasser M, Fauber BP, DeVoss J, Webster J, Diehl L, Modrusan Z, Kirkpatrick DS, Lill JR, Ouyang W, Dixit VM
Nature 2015 Feb 19;518(7539):417-21

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of PPAR delta using 30 µg PC-3 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a PPAR delta polyclonal antibody (Product # PA5-29678) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PPAR delta using 50 µg of mouse brown adipose lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PPAR delta polyclonal antibody (Product # PA5-29678) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PPAR delta using 30 µg of MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PPAR delta polyclonal antibody (Product # PA5-29678) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PPAR delta was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PPAR delta Polyclonal Antibody (Product # PA5-29678) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PPAR delta was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PPAR delta Polyclonal Antibody (Product # PA5-29678) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using PPAR delta (beta) (Product # PA5-29678) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Hepatic mitochondrial dysfunction was not observed in acute IR. (A) Representative haematoxylin and eosin (H&E)-liver sections from CD, HFD-S, and HFD-L mice. Hepatic lipid profiles, including total lipid content (B), total cholesterol (TC, C), and triglyceride (TG, D). (E) Citrate synthase activity in liver tissues from CD, HFD-S, and HFD-L groups. (F) Total ATP content in liver tissues. (G) The mitochondrial DNA (mtDNA) content calculated as the ratio of COX5 to Cyclophilin A DNA levels measured by quantitative PCR in liver tissues. (H) Mitochondrial morphology imaged by transmission electron microscopy (TEM). (I) Complex-dependent respiration determined by electron flow assay in mitochondrial proteins. (J) OCR quantification to measure complex-dependent respiration. (K) OXPHOS complex expression in liver tissues. (L) Complex I activity in liver tissues. (M) Expressions of PGC1 and PPARdelta measured by western blot.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Resistance exercise training-induced PGC-1a4 cooperates with PPARbeta to regulate glycolysis. A PPARbeta mRNA was determined by qPCR in muscle biopsy samples of healthy participants before (SED Pre), within 10 min after (0 h Post), and 1 h after (1 h Post) a one-legged resistance exercise (RE) bout ( n = 16). Time-matched control muscle samples were obtained from the non-exercised leg at 1 h post-RE (SED 1 h Post) ( n = 16 per group). One-way ANOVA was used with multiple comparisons. B Protein abundance of PPARbeta and its downstream related proteins was determined in muscle before (Pre) and after (Post) RET. Representative immunoblots for each protein and quantification of the relative change after training for PPARbeta, NRF-1, MEF2A, and GLUT4 are displayed ( n = 16). Paired two-tailed t -tests were used. C PPARbeta was immunoprecipitated from pooled muscle samples before (pre) and after (post) RET, then the immunoprecipitate was immunoblotted for PGC-1alpha1 and PGC-1alpha4 to determine binding between PPARbeta with PGC-1alpha1 and PGC-1alpha4. Owing to the large amount of protein required to immunoprecipitate enough PPARbeta for immunoblot detection of PGC-1alpha1 and PGC-1alpha4, samples of multiple participants before and after training were pooled together, resulting in a smaller n for PGC-1alpha isoform quantification ( n = 2). D The binding between PPARbeta and PGC-1alpha isoforms was confirmed using cells overexpressing PGC-1alpha4. Experiment shown in panel D was p

PA5-29678

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