PA5-59002

antibody from Invitrogen Antibodies

Targeting: TIMM10 TIM10, TIM10A, TIMM10A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59002

Provider

Invitrogen Antibodies

Product name

TIMM10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MDPLRAQQLA AELEVEMMAD MYNRMTSACH RKCVPPHYKE AELSKGESVC LDRCVSKYLD IHERMGKKLT ELSMQDEELM KRVQQSSGPA

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-TIMM10 Polyclonal Antibody (Product # PA5-59002) and a 10 kDa band corresponding to Mitochondrial import inner membrane translocase subunit Tim10 was observed across all the tested cell lines and tissues. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HEK-293 (Lane 3), Mouse Brain (Lane 4), Mouse Liver (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1/4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TIMM10 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a TIMM10 Polyclonal Antibody (Product # PA5-59002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Mitochondrial import inner membrane translocase subunit Tim10 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TIMM10 Polyclonal Antibody (Product # PA5-59002) at 1/100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1/2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7C1115LZR).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TIMM10 in human kidney using TIMM10 Polyclonal Antibody (Product # PA5-59002) shows strong granular cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TIMM10 in human testis using TIMM10 Polyclonal Antibody (Product # PA5-59002) shows strong granular cytoplasmic positivity.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TIMM10 in human liver using TIMM10 Polyclonal Antibody (Product # PA5-59002) shows moderate to strong granular cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TIMM10 in human heart muscle using TIMM10 Polyclonal Antibody (Product # PA5-59002) shows strong granular cytoplasmic positivity.