PAB23466

antibody from Abnova Corporation

Targeting: TIMM10 TIM10, TIM10A, TIMM10A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23466

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23466, RRID:AB_11121834

Product name

TIMM10 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TIMM10.

Antigen sequence

MDPLRAQQLAAELEVEMMADMYNRMTSACHRKCVP
PHYKEAELSKGESVCLDRCVSKYLDIHERMGKKLT
ELSMQDEELMKRVQQSSGPA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with TIMM10 polyclonal antibody (Cat # PAB23466) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with TIMM10 polyclonal antibody (Cat # PAB23466) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with TIMM10 polyclonal antibody (Cat # PAB23466) shows strong cytoplasmic positivity in cells in tubules at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)