A700-037-T

antibody from Invitrogen Antibodies

Targeting: MED1 CRSP1, CRSP200, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-037-T

Provider

Invitrogen Antibodies

Product name

MED1 Recombinant Rabbit Monoclonal Antibody (BLR037F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR037F

Vial size

20 µL

Concentration

100 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody (BLR037F) (A700-037 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MED1 by western blot. Samples: Whole cell lysate (50 µg) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (Product # A700-037 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED1 by immunocytochemistry.Sample: FFPE section of human HCT 116 cells.Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (Product # A700-037; lot 1) used at 1:100.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse MED1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (Product # A700-037 lot 1) used at 1:500.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED1 by immunohistochemistry.Sample: FFPE section of human small cell lung cancer.Antibody: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (Product # A700-037 lot 1) used at 1:500.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (Product # A700-037 lot 1) used for IP at 20 µL per reaction. MED1 was also immunoprecipitated by rabbit anti-MED1 antibody (Product # A300-793A). For blotting immunoprecipitated MED1 (Product # A700-037) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.