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LearnNB100-2574
antibody from Novus Biologicals
Targeting: MED1
CRSP1, CRSP200, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-2574 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TRAP220/MED1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence.
Brückmann NH, Bennedsen SN, Duijf PHG, Terp MG, Thomassen M, Larsen M, Pedersen CB, Kruse T, Alcaraz N, Ditzel HJ, Gjerstorff MF
Cell death & disease 2019 Nov 6;10(11):841
Cell death & disease 2019 Nov 6;10(11):841
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Detection of Human and Mouse CRSP1(TRAP220) on HeLa whole cell lysate using NB100-2574.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiMED1antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from RAW264.7 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574] - Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED1 antibody NB100-2574(lot NB100-2574-9) used for IP at 6 ug per reaction. For blotting immunoprecipitated MED1, NB100-2574 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:1000 (1ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG.
