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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN950417 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ankylosis, Progressive Homolog (Mouse) (ANKH) (C-Term), (AA 463-492) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 463~492 amino acids from the C-terminal region of human ANKH
- Description
- Affinity chromatography on Protein A
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term,AA 463-492
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The CPPDD-associated ANKH M48T mutation interrupts the interaction of ANKH with the sodium/phosphate cotransporter PiT-1.
Role of the mouse ank gene in control of tissue calcification and arthritis.
Physical map and characterization of transcripts in the candidate interval for familial chondrocalcinosis at chromosome 5p15.1.
Wang J, Tsui HW, Beier F, Tsui FW
The Journal of rheumatology 2009 Jun;36(6):1265-72
The Journal of rheumatology 2009 Jun;36(6):1265-72
Role of the mouse ank gene in control of tissue calcification and arthritis.
Ho AM, Johnson MD, Kingsley DM
Science (New York, N.Y.) 2000 Jul 14;289(5477):265-70
Science (New York, N.Y.) 2000 Jul 14;289(5477):265-70
Physical map and characterization of transcripts in the candidate interval for familial chondrocalcinosis at chromosome 5p15.1.
Rojas K, Serrano de la Peña L, Gallardo T, Simmons A, Nyce K, McGrath R, Considine E, Vasko AJ, Peterson E, Grady D, Cox R, Andrew LJ, Lovett M, Overhauser J, Williams CJ
Genomics 1999 Dec 1;62(2):177-83
Genomics 1999 Dec 1;62(2):177-83
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ANKH Antibody (C-term) (Cat. #AP50180PU-N) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ANKH Antibody (C-term) (Cat. #AP50180PU-N) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ANKH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ANKH Antibody (C-term) (Cat. #AP50180PU-N) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
