Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Proximity ligation assay [1]
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Validation data
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Comment
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- Product number
- H00005906-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005906-M01, RRID:AB_606911
- Product name
- RAP1A monoclonal antibody (M01), clone 3E1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant RAP1A.
- Antigen sequence
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPT
IEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDL
YMKNGQGFALVYSITAQSTFNDLQDLREQILRVKD
TEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCN
CAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKK
PKKKSCLLL- Isotype
- IgG
- Antibody clone number
- 3E1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Overexpression of Rap-1A indicates a poor prognosis for oral cavity squamous cell carcinoma and promotes tumor cell invasion via Aurora-A modulation.
Chen CH, Chuang HC, Huang CC, Fang FM, Huang HY, Tsai HT, Su LJ, Shiu LY, Leu S, Chien CY
The American journal of pathology 2013 Feb;182(2):516-28
The American journal of pathology 2013 Feb;182(2):516-28
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)