NBP2-30087

antibody from Novus Biologicals

Targeting: FLT3 CD135, FLK2, STK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-30087

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Flt-3/Flk-2/CD135 Antibody

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation.

Host

Rabbit

Isotype

IgG

Vial size

0.4 ml

Concentration

1.2 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Flt-3/Flk-2/CD135 Antibody [NBP2-30087] - Western blot analysis of anti-FLT3 Pab (NBP2-30087) in HL-60 cell lysate. FLT3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody [NBP2-30087] - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Flt-3/Flk-2/CD135 Antibody [NBP2-30087] - Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.