Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 14525-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14525-1-AP, RRID:AB_2205881
- Product name
- TOP3A antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TOP3A antibody (Cat. #14525-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder.
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.
The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.
Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2.
Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.
FANCD2, FANCJ and BRCA2 cooperate to promote replication fork recovery independently of the Fanconi Anemia core complex.
TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.
FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks.
FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery.
Jiang W, Jia N, Guo C, Wen J, Wu L, Ogi T, Zhang H
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166106
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166106
The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.
Jiang M, Xie X, Zhu X, Jiang S, Milenkovic D, Misic J, Shi Y, Tandukar N, Li X, Atanassov I, Jenninger L, Hoberg E, Albarran-Gutierrez S, Szilagyi Z, Macao B, Siira SJ, Carelli V, Griffith JD, Gustafsson CM, Nicholls TJ, Filipovska A, Larsson NG, Falkenberg M
Science advances 2021 Jul;7(27)
Science advances 2021 Jul;7(27)
PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.
González-Acosta D, Blanco-Romero E, Ubieto-Capella P, Mutreja K, Míguez S, Llanos S, García F, Muñoz J, Blanco L, Lopes M, Méndez J
The EMBO journal 2021 Jul 15;40(14):e106355
The EMBO journal 2021 Jul 15;40(14):e106355
The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.
Shorrocks AK, Jones SE, Tsukada K, Morrow CA, Belblidia Z, Shen J, Vendrell I, Fischer R, Kessler BM, Blackford AN
Nature communications 2021 Jan 26;12(1):585
Nature communications 2021 Jan 26;12(1):585
Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.
Yang CW, Hsieh MH, Sun HJ, Teng SC
Aging 2021 Apr 4;13(7):10490-10516
Aging 2021 Apr 4;13(7):10490-10516
FANCM suppresses DNA replication stress at ALT telomeres by disrupting TERRA R-loops.
Pan X, Chen Y, Biju B, Ahmed N, Kong J, Goldenberg M, Huang J, Mohan N, Klosek S, Parsa K, Guh CY, Lu R, Pickett HA, Chu HP, Zhang D
Scientific reports 2019 Dec 13;9(1):19110
Scientific reports 2019 Dec 13;9(1):19110
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Dhahrabi HAM, Elcioglu NH, GOSgene., Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesias A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP
American journal of human genetics 2018 Sep 6;103(3):456
American journal of human genetics 2018 Sep 6;103(3):456
Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2.
Hangas A, Aasumets K, Kekäläinen NJ, Paloheinä M, Pohjoismäki JL, Gerhold JM, Goffart S
Nucleic acids research 2018 Oct 12;46(18):9625-9636
Nucleic acids research 2018 Oct 12;46(18):9625-9636
Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson NG, Larsson E, Falkenberg M, Taylor RW, Griffith JD, Gustafsson CM
Molecular cell 2018 Jan 4;69(1):9-23.e6
Molecular cell 2018 Jan 4;69(1):9-23.e6
Mutations in TOP3A Cause a Bloom Syndrome-like Disorder.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Dhahrabi HAM, Elcioglu NH, GOSgene., Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesias A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP
American journal of human genetics 2018 Aug 2;103(2):221-231
American journal of human genetics 2018 Aug 2;103(2):221-231
BLM helicase regulates DNA repair by counteracting RAD51 loading at DNA double-strand break sites.
Patel DS, Misenko SM, Her J, Bunting SF
The Journal of cell biology 2017 Nov 6;216(11):3521-3534
The Journal of cell biology 2017 Nov 6;216(11):3521-3534
RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability.
Chang EY, Novoa CA, Aristizabal MJ, Coulombe Y, Segovia R, Chaturvedi R, Shen Y, Keong C, Tam AS, Jones SJM, Masson JY, Kobor MS, Stirling PC
The Journal of cell biology 2017 Dec 4;216(12):3991-4005
The Journal of cell biology 2017 Dec 4;216(12):3991-4005
FANCD2, FANCJ and BRCA2 cooperate to promote replication fork recovery independently of the Fanconi Anemia core complex.
Raghunandan M, Chaudhury I, Kelich SL, Hanenberg H, Sobeck A
Cell cycle (Georgetown, Tex.) 2015;14(3):342-53
Cell cycle (Georgetown, Tex.) 2015;14(3):342-53
TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.
Blackford AN, Nieminuszczy J, Schwab RA, Galanty Y, Jackson SP, Niedzwiedz W
Molecular cell 2015 Mar 19;57(6):1133-1141
Molecular cell 2015 Mar 19;57(6):1133-1141
FANCD2-controlled chromatin access of the Fanconi-associated nuclease FAN1 is crucial for the recovery of stalled replication forks.
Chaudhury I, Stroik DR, Sobeck A
Molecular and cellular biology 2014 Nov;34(21):3939-54
Molecular and cellular biology 2014 Nov;34(21):3939-54
FANCD2 regulates BLM complex functions independently of FANCI to promote replication fork recovery.
Chaudhury I, Sareen A, Raghunandan M, Sobeck A
Nucleic acids research 2013 Jul;41(13):6444-59
Nucleic acids research 2013 Jul;41(13):6444-59
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Supportive validation
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- Proteintech Group (provider)
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- Experimental details
- The TOP3A antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TOP3A. This antibody recognizes human, mouse, rat antigen. The TOP3A antibody has been validated for the following applications: ELISA, WB analysis.