Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS60749 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- TOP3A polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human TOP3A
- Description
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIÏ« exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIÏ«, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo IIIÉÇ exists as three isoforms,ÉÇ-1,ÉÇ -2 and ÉÇ-3, also produced by alternative splicing. Topo IIIÉÇ-1 is expressed in testis, heart, and skeletal muscle, whereas Topo IIIÉÇ-2 is expressed in thymus, kidney and pancreas.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of TOP3A pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:MCF-7 whole cell lysate