Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- sc-586 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-586, RRID:AB_2223098
- Product name
- Anti-ADCY1
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references CFTR-adenylyl cyclase I association responsible for UTP activation of CFTR in well-differentiated primary human bronchial cell cultures.
Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia.
Index to Volume 38
Clinical Pharmacology and Therapeutics 1985 Dec;38(6):707-711
Namkung W, Finkbeiner WE, Verkman AS
Molecular biology of the cell 2010 Aug 1;21(15):2639-48
Molecular biology of the cell 2010 Aug 1;21(15):2639-48
Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia.
Nlend MC, Schmid A, Sutto Z, Ransford GA, Conner GE, Fregien N, Salathe M
FEBS letters 2007 Jul 10;581(17):3241-6
FEBS letters 2007 Jul 10;581(17):3241-6
Index to Volume 38
Clinical Pharmacology and Therapeutics 1985 Dec;38(6):707-711
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 123
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human nasopharynx shows strong nuclear positivity in respiratory epithelial cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.