sc-586

antibody from Santa Cruz Biotechnology

Targeting: ADCY1 AC1, DFNB44

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-586

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-586, RRID:AB_2223098

Product name

Anti-ADCY1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

CFTR-adenylyl cyclase I association responsible for UTP activation of CFTR in well-differentiated primary human bronchial cell cultures.

Namkung W, Finkbeiner WE, Verkman AS
Molecular biology of the cell 2010 Aug 1;21(15):2639-48

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Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia.

Nlend MC, Schmid A, Sutto Z, Ransford GA, Conner GE, Fregien N, Salathe M
FEBS letters 2007 Jul 10;581(17):3241-6

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Index to Volume 38

Clinical Pharmacology and Therapeutics 1985 Dec;38(6):707-711

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 123

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human nasopharynx shows strong nuclear positivity in respiratory epithelial cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.