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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- UM500065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERG Monoclonal Antibody (UMAB77), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB77
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35 µg) from 8 different cell lines (1: HeLa, 2: SV-T2, 3: A549. 4: COS7, 5: Jurkat, 6: MDCK, 7: PC-12, 8: MCF7).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-ERG mouse monoclonal antibody (UM500065, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IHC staining of paraffin-embedded carcinoma of human prostate using anti-ERG clone UMAB77 at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500056 requires heat-induced epitope retrieval with EDTA (pH8.7) at 95-100C 20 min. The composit image shows 3 prostate tumors with no staining in tumor cells of figure A and B altho µgh strong nuclear staining is present in the endothelial cells. Figure C shows strong nuclear with weak cytoplasmic staining in prostate tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-ERG clone UMAB77 at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500065 requires heat-induced epitope retrieval with ACCEL (pH8.7) biocare pressure cooker. The image shows prostate tumors staining in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-ERG mouse monoclonal antibody. (UM500065; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERG mouse monoclonal antibody (UM500065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERG mouse monoclonal antibody (UM500065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
