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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-731 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-731, RRID:AB_2217962
- Product name
- Anti-YWHAG
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.
Characterization of the Brain 26S Proteasome and its Interacting Proteins.
Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms.
14-3-3zeta contributes to tyrosine hydroxylase activity in MN9D cells: localization of dopamine regulatory proteins to mitochondria.
14-3-3{eta} Amplifies Androgen Receptor Actions in Prostate Cancer.
Presence and distribution of 14-3-3 proteins in human ocular surface tissues.
Okamoto Y, Shirakashi Y, Ihara M, Urushitani M, Oono M, Kawamoto Y, Yamashita H, Shimohama S, Kato S, Hirano A, Tomimoto H, Ito H, Takahashi R
PloS one 2011;6(5):e20427
PloS one 2011;6(5):e20427
Characterization of the Brain 26S Proteasome and its Interacting Proteins.
Tai HC, Besche H, Goldberg AL, Schuman EM
Frontiers in molecular neuroscience 2010;3
Frontiers in molecular neuroscience 2010;3
Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms.
Rajagopalan S, Sade RS, Townsley FM, Fersht AR
Nucleic acids research 2010 Jan;38(3):893-906
Nucleic acids research 2010 Jan;38(3):893-906
14-3-3zeta contributes to tyrosine hydroxylase activity in MN9D cells: localization of dopamine regulatory proteins to mitochondria.
Wang J, Lou H, Pedersen CJ, Smith AD, Perez RG
The Journal of biological chemistry 2009 May 22;284(21):14011-9
The Journal of biological chemistry 2009 May 22;284(21):14011-9
14-3-3{eta} Amplifies Androgen Receptor Actions in Prostate Cancer.
Titus MA, Tan JA, Gregory CW, Ford OH, Subramanian RR, Fu H, Wilson EM, Mohler JL, French FS
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Dec 15;15(24):7571-7581
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Dec 15;15(24):7571-7581
Presence and distribution of 14-3-3 proteins in human ocular surface tissues.
Shankardas J, Senchyna M, Dimitrijevich SD
Molecular vision 2008;14:2604-15
Molecular vision 2008;14:2604-15
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human breast shows nuclear and cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
