GTX110629

antibody from GeneTex

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110629

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110629, RRID:AB_1952631

Product name

14-3-3 gamma antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX110629 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 12% SDS PAGE GTX110629 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 gamma antibody (GTX110629) diluted at 1:1000 and competitor's antibody (sc-731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 gamma antibody (GTX110629) diluted at 1:1000 and competitor's antibody (sc-731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX110629) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using 14-3-3 gamma(GTX110629) antibody at 1:500 dilution.