NB100-407

antibody from Novus Biologicals

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NB100-407

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-407, RRID:AB_10003122

Product name

Mouse Monoclonal 14-3-3 gamma Antibody

Antibody type

Monoclonal

Description

Unpurified. NB100-407 reacts with the human form of intact, non-modified N-terminus 14-3-3 gamma. The naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Zebrafish

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

MetAP2 inhibition increases energy expenditure through direct action on brown adipocytes.

Huang HJ, Holub C, Rolzin P, Bilakovics J, Fanjul A, Satomi Y, Plonowski A, Larson CJ, Farrell PJ
The Journal of biological chemistry 2019 Jun 14;294(24):9567-9575

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Decoding the Function of Expansion Segments in Ribosomes.

Fujii K, Susanto TT, Saurabh S, Barna M
Molecular cell 2018 Dec 20;72(6):1013-1020.e6

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Omics Assisted N-terminal Proteoform and Protein Expression Profiling On Methionine Aminopeptidase 1 (MetAP1) Deletion.

Jonckheere V, Fijałkowska D, Van Damme P
Molecular & cellular proteomics : MCP 2018 Apr;17(4):694-708

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Phosphorylation dependence and stoichiometry of the complex formed by tyrosine hydroxylase and 14-3-3γ.

Kleppe R, Rosati S, Jorge-Finnigan A, Alvira S, Ghorbani S, Haavik J, Valpuesta JM, Heck AJ, Martinez A
Molecular & cellular proteomics : MCP 2014 Aug;13(8):2017-30

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Pyridinylquinazolines selectively inhibit human methionine aminopeptidase-1 in cells.

Zhang F, Bhat S, Gabelli SB, Chen X, Miller MS, Nacev BA, Cheng YL, Meyers DJ, Tenney K, Shim JS, Crews P, Amzel LM, Ma D, Liu JO
Journal of medicinal chemistry 2013 May 23;56(10):3996-4016

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Substituted oxines inhibit endothelial cell proliferation and angiogenesis.

Bhat S, Shim JS, Zhang F, Chong CR, Liu JO
Organic & biomolecular chemistry 2012 Apr 21;10(15):2979-92

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Effect of nitroxoline on angiogenesis and growth of human bladder cancer.

Shim JS, Matsui Y, Bhat S, Nacev BA, Xu J, Bhang HE, Dhara S, Han KC, Chong CR, Pomper MG, So A, Liu JO
Journal of the National Cancer Institute 2010 Dec 15;102(24):1855-73

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Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression.

Hu X, Addlagatta A, Lu J, Matthews BW, Liu JO
Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 28;103(48):18148-53

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).