NBP2-50579

antibody from Novus Biologicals

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-50579

Provider

Novus Biologicals

Product name

Mouse Monoclonal 14-3-3 gamma Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ul

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate