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Western blot
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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB2048 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2048, RRID:AB_1706673
- Product name
- YWHAG monoclonal antibody, clone J3H10
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein corresponding to amino acids 1-247 of human YWHAG.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 μl
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references 14-3-3gamma is upregulated by in vitro ischemia and binds to protein kinase Raf in primary cultures of astrocytes.
The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes.
Chen XQ, Chen JG, Zhang Y, Hsiao WW, Yu AC
Glia 2003 Jun;42(4):315-24
Glia 2003 Jun;42(4):315-24
The association of 14-3-3gamma and actin plays a role in cell division and apoptosis in astrocytes.
Chen XQ, Yu AC
Biochemical and biophysical research communications 2002 Aug 23;296(3):657-63
Biochemical and biophysical research communications 2002 Aug 23;296(3):657-63
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- The lysates of mouse brain (lane 1, 30 ug) and HeLa (lane 2, 30 ug) were resolved by SDS-PAGE and probed with YWHAG monoclonal antibody, clone J3H10 (Cat # MAB2048) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
