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12381-1-AP

antibody from Invitrogen Antibodies

Targeting: YWHAG 14-3-3GAMMA, 14-3-3γ, PPP1R170

Provider product page for 12381-1-AP

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [8]
Immunocytochemistry [1]
Immunohistochemistry [4]
Other assay [1]

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Validation data

Product number: 12381-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: 14-3-3 gamma Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Reactivity: Human, Mouse, Rat, Canine
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.42 mg/mL
Storage: -20°C

YWHAG protein structure - 12381-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human brain tissue were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: U-937 cells were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RNAi result of 12381-1-AP (anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 12381-1-AP ( 14-3-3 GAMMA-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.