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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AM09044PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09044PU-S, RRID:AB_1609704
- Product name
- anti 14-3-3 protein gamma
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human 14-3-3 gamma (1-247 aa) purified from E. coli (NP_036611).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- J3H10
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of HeLa (30 ug) and mouse brain (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-Human 14-3-3 gamma antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of HeLa(30ug) and mouse brain(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 gamma (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09044PU-N 14-3-3 gamma antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09044PU-N 14-3-3 gamma antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
