FNab03293

antibody from FineTest Biotech
Targeting: PPP1R15A GADD34
Provider product page for FNab03293
Western blot
ELISA
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
FNab03293 - Provider product page
Provider
FineTest Biotech
Product name
GADD34 antibody
Antibody type
Polyclonal
Antigen
Recombinant protein fragment
Description
GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4(ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase(PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 appears to be about 100 kDa which was confirmed in GADD34 KO mice and drug treated cells, despite the predicated MW of 73 kDa, and this antibody primarily recongize the 100 kDa band.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Conjugate
Unconjugated
Antigen sequence
protein phosphatase 1, regulatory(i
nhibitor) subunit 15A
Epitope
IgG
Isotype
IgG
Vial size
100¦Ìg
Concentration
Immunogen affinity purified
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
Handling
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)
PPP1R15A protein structure - FNab03293 shown in red.