Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-01787 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GADD34 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.25 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.
Rivera-Reyes A, Ye S, E Marino G, Egolf S, E Ciotti G, Chor S, Liu Y, Posimo JM, Park PMC, Pak K, Babichev Y, Sostre-Colón J, Tameire F, Leli NM, Koumenis C, C Brady D, Mancuso A, Weber K, Gladdy R, Qi J, Eisinger-Mathason TSK
Cell death & disease 2018 Oct 31;9(11):1108
Cell death & disease 2018 Oct 31;9(11):1108
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD34 Antibody (3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD34 Antibody (3D12) [NBP2-01787] Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GADD34 Antibody (3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GADD34 Antibody (3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).