A02916

antibody from Boster Biological Technology

Targeting: MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A02916

Provider

Boster Biological Technology

Product name

Anti-MKK3 (Ab-189) MAP2K3 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for P46734 detection. Tested positive for WB IHC IF in Hu Ms

Reactivity

Human, Mouse

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.

References

Submitted references

Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway.

Cheng H, Wang W, Wang G, Wang A, Du L, Lou W
Medical science monitor : international medical journal of experimental and clinical research 2018 Feb 7;24:768-781

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of MAP2K3 using anti-MAP2K3 antibody (A02916).MAP2K3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K3 Antibody (A02916) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image