Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ7194 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NOS1AP Antibody / CAPON
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NOS1AP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEL, 2) human A431, 3) human HaCaT, 4) human MCF7, 5) rat brain and 6) mouse brain tissue lysate with NOS1AP antibody. Predicted molecular weight ~56 kDa but ca be observed at ~70 kDa (NOS1AP-L) and 95-100 kDa (NOS1APc).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with NOS1AP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HEL cells with NOS1AP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOS1AP antibody.