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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3601 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3601, RRID:AB_1574970
- Product name
- FREQ polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FREQ.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Up-regulation of neuronal calcium sensor-1 (NCS-1) in the prefrontal cortex of schizophrenic and bipolar patients.
The neuronal calcium sensor family of Ca2+-binding proteins.
Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1).
Koh PO, Undie AS, Kabbani N, Levenson R, Goldman-Rakic PS, Lidow MS
Proceedings of the National Academy of Sciences of the United States of America 2003 Jan 7;100(1):313-7
Proceedings of the National Academy of Sciences of the United States of America 2003 Jan 7;100(1):313-7
The neuronal calcium sensor family of Ca2+-binding proteins.
Burgoyne RD, Weiss JL
The Biochemical journal 2001 Jan 1;353(Pt 1):1-12
The Biochemical journal 2001 Jan 1;353(Pt 1):1-12
Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1).
Bourne Y, Dannenberg J, Pollmann V, Marchot P, Pongs O
The Journal of biological chemistry 2001 Apr 13;276(15):11949-55
The Journal of biological chemistry 2001 Apr 13;276(15):11949-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MCF-7 cell lysate (35 ug/lane) with FREQ polyclonal antibody (Cat # PAB3601).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinomareacted with FREQ polyclonal antibody (Cat # PAB3601), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ZR-75-1 cells using FREQ polyclonal antibody (Cat # PAB3601)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
