NBP1-89820

antibody from Novus Biologicals

Targeting: NCS1 FREQ, NCS-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89820

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89820, RRID:AB_11022727

Product name

Rabbit Polyclonal Frequenin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse Frequenin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Frequenin Antibody [NBP1-89820] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Frequenin Antibody [NBP1-89820] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Frequenin Antibody [NBP1-89820] - Analysis in control (vector only transfected HEK293T lysate) and NCS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human cerebral cortex shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Frequenin Antibody [NBP1-89820] - Staining in human cerebral cortex and liver tissues using anti-NCS1 antibody. Corresponding NCS1 RNA-seq data are presented for the same tissues.