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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-59617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCDC86 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LQQGAGLESP QGQPEPGAAS PQRQQDLHLE SPQRQPEYSP ESPRCQPKPS EEAPKCSQDQ GVLASELAQN KEELTPGAPQ HQLPPV
- Concentration
- 0.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCDC86 in control (vector only transfected HEK293T lysate) and CCDC86 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a CCDC86 Polyclonal Antibody (Product # PA5-59617).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CCDC86 in human cell line U-2 OS shows positivity in nucleus & nucleoli. Samples were probed using a CCDC86 Polyclonal Antibody (Product # PA5-59617).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CCDC86 in human cell line A-431 using a CCDC86 Polyclonal Antibody (Product # PA5-59617) shows localization to nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human colon using CCDC86 Polyclonal Antibody (Product # PA5-59617).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human kidney using CCDC86 Polyclonal Antibody (Product # PA5-59617).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human parathyroid gland and liver tissues using CCDC86 Polyclonal Antibody (Product # PA5-59617). Corresponding CCDC86 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human colon, kidney, liver and parathyroid gland using CCDC86 Polyclonal Antibody (Product # PA5-59617) (A) shows similar protein distribution across tissues to an independent CCDC86 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human parathyroid gland using CCDC86 Polyclonal Antibody (Product # PA5-59617) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC86 in human liver using CCDC86 Polyclonal Antibody (Product # PA5-59617) shows low expression as expected.
