PAB23751

antibody from Abnova Corporation

Targeting: CCDC86 MGC2574

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23751

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23751, RRID:AB_11123685

Product name

CCDC86 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CCDC86.

Antigen sequence

LEEEKERRRQEKKQRRAENLKRRLENERKAEVVQV
IRNPAKLKRAKKKQLRSIEKRDTLALLQKQPPQQP
AAKI


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with CCDC86 polyclonal antibody (Cat # PAB23751).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CCDC86 polyclonal antibody (Cat # PAB23751) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus with CCDC86 polyclonal antibody (Cat # PAB23751) shows strong nuclear positivity in squamous epithelial cells at 1:1000-1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)