Antibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11204-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11204-1-AP, RRID:AB_2099064
- Product name
- ENO1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ENO1 antibody (Cat. #11204-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy.
lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1.
Long non-coding RNA CTSLP8 mediates ovarian cancer progression and chemotherapy resistance by modulating cellular glycolysis and regulating c-Myc expression through PKM2.
Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.
Knockdown of GTF2E2 inhibits the growth and progression of lung adenocarcinoma via RPS4X in vitro and in vivo.
FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia.
Valproic acid Suppresses Breast Cancer Cell Growth Through Triggering Pyruvate Kinase M2 Isoform Mediated Warburg Effect.
Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Fast and unbiased purification of RNA-protein complexes after UV cross-linking.
Targeting G6PD reverses paclitaxel resistance in ovarian cancer by suppressing GSTP1.
Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells.
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients.
Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.
Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.
Functional Effects of Cigarette Smoke-Induced Changes in Airway Smooth Muscle Mitochondrial Morphology.
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
Enolase 1 stimulates glycolysis to promote chemoresistance in gastric cancer.
Defining the Na+/H+ exchanger NHE1 interactome in triple-negative breast cancer cells.
Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.
FBXW7 negatively regulates ENO1 expression and function in colorectal cancer.
Alpha-enolase promotes cell glycolysis, growth, migration, and invasion in non-small cell lung cancer through FAK-mediated PI3K/AKT pathway.
Enolase-1 is a therapeutic target in endometrial carcinoma.
α-Enolase plays a catalytically independent role in doxorubicin-induced cardiomyocyte apoptosis and mitochondrial dysfunction.
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.
Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma.
Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.
Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.
Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.
Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.
Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma.
Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.
Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.
Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.
Lu L, Li X, Zhong Z, Zhou W, Zhou D, Zhu M, Miao C
International journal of biological sciences 2021;17(15):4093-4107
International journal of biological sciences 2021;17(15):4093-4107
lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1.
Luo J, Wang H, Wang L, Wang G, Yao Y, Xie K, Li X, Xu L, Shen Y, Ren B
Molecular therapy. Nucleic acids 2021 Sep 3;25:11-24
Molecular therapy. Nucleic acids 2021 Sep 3;25:11-24
Long non-coding RNA CTSLP8 mediates ovarian cancer progression and chemotherapy resistance by modulating cellular glycolysis and regulating c-Myc expression through PKM2.
Li X, Zhang Y, Wang X, Lin F, Cheng X, Wang Z, Wang X
Cell biology and toxicology 2021 Sep 12;
Cell biology and toxicology 2021 Sep 12;
Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.
Li R, Li H, Zhu L, Zhang X, Liu D, Li Q, Ni B, Hu L, Zhang Z, Zhang Y, Wang X, Jiang SH
Cell death & disease 2021 Nov 26;12(12):1106
Cell death & disease 2021 Nov 26;12(12):1106
Knockdown of GTF2E2 inhibits the growth and progression of lung adenocarcinoma via RPS4X in vitro and in vivo.
Bi G, Zhu D, Bian Y, Huang Y, Zhan C, Yang Y, Wang Q
Cancer cell international 2021 Mar 23;21(1):181
Cancer cell international 2021 Mar 23;21(1):181
FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.
Na Y, Kim H, Choi Y, Shin S, Jung JH, Kwon SC, Kim VN, Kim JS
Nucleic acids research 2021 Mar 18;49(5):e28
Nucleic acids research 2021 Mar 18;49(5):e28
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Wang XH, Xu S, Zhou XY, Zhao R, Lin Y, Cao J, Zang WD, Tao H, Xu W, Li MQ, Zhao SM, Jin LP, Zhao JY
Nature communications 2021 Jun 8;12(1):3428
Nature communications 2021 Jun 8;12(1):3428
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia.
Wei L, Zhong W, Sun T, Li H, Sun T, Han Y, Sun D, Li X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jan;133:110998
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jan;133:110998
Valproic acid Suppresses Breast Cancer Cell Growth Through Triggering Pyruvate Kinase M2 Isoform Mediated Warburg Effect.
Li Z, Yang L, Zhang S, Song J, Sun H, Shan C, Wang D, Liu S
Cell transplantation 2021 Jan-Dec;30:9636897211027524
Cell transplantation 2021 Jan-Dec;30:9636897211027524
Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.
Maitituoheti M, Keung EZ, Tang M, Yan L, Alam H, Han G, Singh AK, Raman AT, Terranova C, Sarkar S, Orouji E, Amin SB, Sharma S, Williams M, Samant NS, Dhamdhere M, Zheng N, Shah T, Shah A, Axelrad JB, Anvar NE, Lin YH, Jiang S, Chang EQ, Ingram DR, Wang WL, Lazar A, Lee MG, Muller F, Wang L, Ying H, Rai K
Cell reports 2020 Oct 20;33(3):108293
Cell reports 2020 Oct 20;33(3):108293
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Li ST, Huang, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269
Nature metabolism 2020 Mar;2(3):256-269
Fast and unbiased purification of RNA-protein complexes after UV cross-linking.
Urdaneta EC, Beckmann BM
Methods (San Diego, Calif.) 2020 Jun 1;178:72-82
Methods (San Diego, Calif.) 2020 Jun 1;178:72-82
Targeting G6PD reverses paclitaxel resistance in ovarian cancer by suppressing GSTP1.
Feng Q, Li X, Sun W, Sun M, Li Z, Sheng H, Xie F, Zhang S, Shan C
Biochemical pharmacology 2020 Aug;178:114092
Biochemical pharmacology 2020 Aug;178:114092
Accelerated Metabolite Levels of Aerobic Glycolysis and the Pentose Phosphate Pathway Are Required for Efficient Replication of Infectious Spleen and Kidney Necrosis Virus in Chinese Perch Brain Cells.
Guo X, Wu S, Li N, Lin Q, Liu L, Liang H, Niu Y, Huang Z, Fu X
Biomolecules 2019 Sep 2;9(9)
Biomolecules 2019 Sep 2;9(9)
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
Urdaneta EC, Vieira-Vieira CH, Hick T, Wessels HH, Figini D, Moschall R, Medenbach J, Ohler U, Granneman S, Selbach M, Beckmann BM
Nature communications 2019 Mar 1;10(1):990
Nature communications 2019 Mar 1;10(1):990
Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients.
Zhu W, Li H, Yu Y, Chen J, Chen X, Ren F, Ren Z, Cui G
Cancer management and research 2018;10:5735-5745
Cancer management and research 2018;10:5735-5745
Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.
Owusu L, Xu C, Chen H, Liu G, Zhang G, Zhang J, Tang Z, Sun Z, Yi X
Journal of molecular histology 2018 Aug;49(4):347-356
Journal of molecular histology 2018 Aug;49(4):347-356
Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.
Gao S, Liu XP, Wei LH, Lu J, Liu P
Canadian journal of physiology and pharmacology 2018 Apr;96(4):352-358
Canadian journal of physiology and pharmacology 2018 Apr;96(4):352-358
Functional Effects of Cigarette Smoke-Induced Changes in Airway Smooth Muscle Mitochondrial Morphology.
Aravamudan B, Thompson M, Sieck GC, Vassallo R, Pabelick CM, Prakash YS
Journal of cellular physiology 2017 May;232(5):1053-1068
Journal of cellular physiology 2017 May;232(5):1053-1068
CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.
Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825
EMBO reports 2017 May;18(5):809-825
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347
The EMBO journal 2017 May 15;36(10):1330-1347
Enolase 1 stimulates glycolysis to promote chemoresistance in gastric cancer.
Qian X, Xu W, Xu J, Shi Q, Li J, Weng Y, Jiang Z, Feng L, Wang X, Zhou J, Jin H
Oncotarget 2017 Jul 18;8(29):47691-47708
Oncotarget 2017 Jul 18;8(29):47691-47708
Defining the Na+/H+ exchanger NHE1 interactome in triple-negative breast cancer cells.
Amith SR, Vincent KM, Wilkinson JM, Postovit LM, Fliegel L
Cellular signalling 2017 Jan;29:69-77
Cellular signalling 2017 Jan;29:69-77
Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.
Sun B, Bai Y, Zhang L, Gong L, Qi X, Li H, Wang F, Chi X, Jiang Y, Shao S
PloS one 2016;11(9):e0163622
PloS one 2016;11(9):e0163622
FBXW7 negatively regulates ENO1 expression and function in colorectal cancer.
Zhan P, Wang Y, Zhao S, Liu C, Wang Y, Wen M, Mao JH, Wei G, Zhang P
Laboratory investigation; a journal of technical methods and pathology 2015 Sep;95(9):995-1004
Laboratory investigation; a journal of technical methods and pathology 2015 Sep;95(9):995-1004
Alpha-enolase promotes cell glycolysis, growth, migration, and invasion in non-small cell lung cancer through FAK-mediated PI3K/AKT pathway.
Fu QF, Liu Y, Fan Y, Hua SN, Qu HY, Dong SW, Li RL, Zhao MY, Zhen Y, Yu XL, Chen YY, Luo RC, Li R, Li LB, Deng XJ, Fang WY, Liu Z, Song X
Journal of hematology & oncology 2015 Mar 8;8:22
Journal of hematology & oncology 2015 Mar 8;8:22
Enolase-1 is a therapeutic target in endometrial carcinoma.
Zhao M, Fang W, Wang Y, Guo S, Shu L, Wang L, Chen Y, Fu Q, Liu Y, Hua S, Fan Y, Liu Y, Deng X, Luo R, Mei Z, Jiang Q, Liu Z
Oncotarget 2015 Jun 20;6(17):15610-27
Oncotarget 2015 Jun 20;6(17):15610-27
α-Enolase plays a catalytically independent role in doxorubicin-induced cardiomyocyte apoptosis and mitochondrial dysfunction.
Gao S, Li H, Feng XJ, Li M, Liu ZP, Cai Y, Lu J, Huang XY, Wang JJ, Li Q, Chen SR, Ye JT, Liu PQ
Journal of molecular and cellular cardiology 2015 Feb;79:92-103
Journal of molecular and cellular cardiology 2015 Feb;79:92-103
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44
Cell research 2015 Apr;25(4):429-44
Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.
Ma X, Li C, Sun L, Huang D, Li T, He X, Wu G, Yang Z, Zhong X, Song L, Gao P, Zhang H
Nature communications 2014 Oct 10;5:5212
Nature communications 2014 Oct 10;5:5212
Alpha-enolase as a potential cancer prognostic marker promotes cell growth, migration, and invasion in glioma.
Song Y, Luo Q, Long H, Hu Z, Que T, Zhang X, Li Z, Wang G, Yi L, Liu Z, Fang W, Qi S
Molecular cancer 2014 Mar 21;13:65
Molecular cancer 2014 Mar 21;13:65
Mitochondrial binding of α-enolase stabilizes mitochondrial membrane: its role in doxorubicin-induced cardiomyocyte apoptosis.
Gao S, Li H, Cai Y, Ye JT, Liu ZP, Lu J, Huang XY, Feng XJ, Gao H, Chen SR, Li M, Liu PQ
Archives of biochemistry and biophysics 2014 Jan 15;542:46-55
Archives of biochemistry and biophysics 2014 Jan 15;542:46-55
Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells.
Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ
Journal of agricultural and food chemistry 2014 Dec 3;62(48):11672-80
Journal of agricultural and food chemistry 2014 Dec 3;62(48):11672-80
Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.
Choi M, Choi JW, Chaudhari HN, Aseer KR, Mukherjee R, Yun JW
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;31(2-3):408-20
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;31(2-3):408-20
Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.
Liu CI, Wang RY, Lin JJ, Su JH, Chiu CC, Chen JC, Chen JY, Wu YJ
Journal of proteomics 2012 Oct 22;75(18):5578-89
Journal of proteomics 2012 Oct 22;75(18):5578-89
Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma.
Liu Z, Chen C, Yang H, Zhang Y, Long J, Long X, Fang W
Proteomics 2012 Nov;12(22):3416-25
Proteomics 2012 Nov;12(22):3416-25
Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.
Liu C, Zhang A, Guo J, Yang J, Zhou H, Chen H, Jin M
Journal of proteome research 2012 Nov 2;11(11):5396-405
Journal of proteome research 2012 Nov 2;11(11):5396-405
Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.
Bai Z, Ye Y, Liang B, Xu F, Zhang H, Zhang Y, Peng J, Shen D, Cui Z, Zhang Z, Wang S
International journal of oncology 2011 Feb;38(2):375-83
International journal of oncology 2011 Feb;38(2):375-83
Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.
Lu Y, Guo J, Di Y, Zong Y, Qu S, Tian J
Molecules and cells 2009 Sep;28(3):175-81
Molecules and cells 2009 Sep;28(3):175-81
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP(ENO1 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ENO1 antibody 11204-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ENO1 (IP:11204-1-AP, 3ug; Detection:11204-1-AP 1:500) with mouse skeletal muscle tissue lysate 3500ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 11204-1-AP(ENO1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue