LS-C331216

antibody from LSBio

Targeting: ENO1 ENO1L1, MBP-1, MPB1, PPH

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C331216

Provider

LSBio

Product name

ENO1 / Alpha Enolase Antibody LS-C331216

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibodyat 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibodyat a dilition of 1:1000.