F54858

antibody from NSJ Bioreagents

Targeting: ENO1 ENO1L1, MBP-1, MPB1, PPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54858

Provider

NSJ Bioreagents

Product name

ENO1 Antibody / Enolase 1 / Alpha Enolase

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.08 µl, 0.4 µl

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the ENO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251, 4) mouse NIH 3T3 and 5) mouse brain lysate with ENO1 antibody. Predicted molecular weight ~47 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized mouse C2C12 cells with ENO1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lymph tissue with ENO1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with ENO1 antibody; Left=isotype control, Right= ENO1 antibody.