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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-54821 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCRIB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VSVIQFLEAP IGDEDAEEAA AEKRGLQRRA TPHPSELKVM KRSIEGRRSE ACPCQPDSGS PLPAEEEKRL SAESGLSEDS RPSASTVS
- Concentration
- 0.35 mg/mL
Submitted references Quantitative fragmentomics allow affinity mapping of interactomes.
Gogl G, Zambo B, Kostmann C, Cousido-Siah A, Morlet B, Durbesson F, Negroni L, Eberling P, Jané P, Nominé Y, Zeke A, Østergaard S, Monsellier É, Vincentelli R, Travé G
Nature communications 2022 Sep 17;13(1):5472
Nature communications 2022 Sep 17;13(1):5472
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SCRIB in human cell lines MCF-7 and Caco-2 using a SCRIB Polyclonal Antibody (Product # PA5-54821). Corresponding SCRIB RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SCRIB in human cell line U-2 OS shows positivity in plasma membrane, cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a SCRIB Polyclonal Antibody (Product # PA5-54821).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SCRIB in human skin and liver tissues using SCRIB Polyclonal Antibody (Product # PA5-54821). Corresponding SCRIB RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SCRIB in human skin using SCRIB Polyclonal Antibody (Product # PA5-54821) shows moderate membranous positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SCRIB in human duodenum using SCRIB Polyclonal Antibody (Product # PA5-54821) shows moderate membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SCRIB in human cerebral cortex using SCRIB Polyclonal Antibody (Product # PA5-54821) shows moderate to strong cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SCRIB in human liver using SCRIB Polyclonal Antibody (Product # PA5-54821) shows weak membranous positivity in hepatocytes.
