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Learn13241-1-AP
antibody from Invitrogen Antibodies
Targeting: CYP1A1
CP11, CYP1, P1-450, P450-C, P450DX
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 13241-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ISNGQSMSF SPDSGPVWAA RRRLAQNGLK SFSIASDPAS STSCYLEEHV SKEAEVLIST LQELMAGPGH FNPYRYVVVS VTNVICAICF GRRYDHNHQE LLSLVNLNNN FGEVVGSGNP ADFIPILRYL PNPSLNAFKD LNEKFYSFMQ KMVKEHYKTF EKGHIRDITD SLIEHCQEKQ LDENANVQLS DEKIINIVLD LFGAGFDTVT TAISWSLMYL VMNPRVQRKI QEELDTVIGR SRRPRLSDRS HLPYMEAFIL ETFRHSSFVP FTIPHSTTRD TSLKGFYIPK GRCVFVNQWQ INHDQKLWVN PSEFLPERFL TPDGAIDKVL SEKVIIFGMG KRKCIGETIA RW (115-465 aa encoded by BC023019)
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CYP1A1 antibody (13241-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP1A1 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 13241-1-AP (CYP1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13241-1-AP ( CYP1A1 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13241-1-AP ( CYP1A1 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were stained with 0.5ug CYP1A1 antibody (13241-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CYP1A1 (IP:13241-1-AP, 4ug; Detection:13241-1-AP 1:1000) with mouse lung tissue lysate 3600ug.
