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LearnNBP2-12897
antibody from Novus Biologicals
Targeting: KCNQ1
JLNS1, KCNA8, KCNA9, Kv7.1, KVLQT1, LQT, LQT1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP2-12897 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Kv7.1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 75kDa.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kv7.1 Antibody (S37A-10) [NBP2-12897] - analysis of hamster T-CHO cell lysate showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kv7.1 Antibody (S37A-10) [NBP2-12897] - analysis of Human Cell lysates showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A-10 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Kv7.1 Antibody (S37A-10) [NBP2-12897] - Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Kv7.1 Antibody (S37A-10) [NBP2-12897] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
