Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP1-77776 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77776, RRID:AB_11037080
- Product name
- Rabbit Polyclonal SEPHS2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 2.3 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: SEPHS2 Antibody [NBP1-77776] - Shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.