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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 200-401-999 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-999, RRID:AB_2194568
- Product name
- Anti-Selenophosphate Synthetase 2 (SPS2) (RABBIT) Antibody - 200-401-999
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease.
Pitts MW, Kremer PM, Hashimoto AC, Torres DJ, Byrns CN, Williams CS, Berry MJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 18;35(46):15326-38
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 18;35(46):15326-38
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
- Validation comment
- Western Blot
