AP6579a

antibody from Abgent

Targeting: SERPINB3 HsT1196, SCC, SCCA1, T4-A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6579a

Provider

Abgent

Proper citation

Abgent Cat#AP6579a, RRID:AB_1968276

Product name

SPB3 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of SPB3 antibody (N-term) (Cat. #AP6579a) in 293 cell line lysates (35ug/lane). SPB3 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SPB3 Antibody (N-term) (Cat. #AP6579a) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

SPB3 Antibody (N-term) (Cat. #AP6579a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50