231-MSM1-P1

antibody from Invitrogen Antibodies

Targeting: AKR1B1 ALDR1, AR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

231-MSM1-P1

Provider

Invitrogen Antibodies

Product name

Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (CPTC-AKR1B1-3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CPTC-AKR1B1-3

Vial size

100 µg

Concentration

200 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Human Kidney tissue, Human HepG2, HEK293, HeLa and Jurkat cell lysate using Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (Product # 231-MSM1-P1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of A549 cells using Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (Product # 231-MSM1-P1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot (Red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human prostate tumor using Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (Product # 231-MSM1-P1).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (Product # 231-MSM1-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDís) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDís) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analyis Purified Aldo-keto Reductase Family 1 Member B1 Monoclonal Antibody (Product # 231-MSM1-P1). Confirmation of Purity and Integrity of Antibody.