HPA036089

antibody from Atlas Antibodies

Targeting: TNS1 DKFZp586K0617, MXRA6, PPP1R155, TNS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA036089

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA036089, RRID:AB_10796111

Product name

Anti-TNS1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAH
AGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTT
SHYAHDPSGMFRSQS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TNS1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and A-431 using Anti-TNS1 antibody. Corresponding TNS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-2 OS

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in tubules and cells in glomeruli.

Sample type

HUMAN