10513-1-AP

antibody from Invitrogen Antibodies

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Immunoprecipitation

Antibody data

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Validation data

Product number: 10513-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: MCM2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: FDILCVVRD TVDPVQDEML ARFVVGSHVR HHPSNKEEEG LANGSAAEPA MPNTYGVEPL PQEVLKKYII YAKERVHPKL NQMDQDKVAK MYSDLRKESM ATGSIPITVR HIESMIRMAE AHARIHLRDY VIEDDVNMAI RVMLESFIDT QKFSVMRSMR KTFARYLSFR RDNNELLLFI LKQLVAEQVT YQRNRFGAQQ DTIEVPEKDL VDKARQINIH NLSAFYDSEL FRMNKFSHDL KRKMILQQF (657-904 aa encoded by BC007670)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.27 mg/mL
Storage: -20°C

MCM2 protein structure - 10513-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PC-3 cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ( 4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human placenta tissue slide using 10513-1-AP ( MCM2 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP ( MCM2 Antibody) at dilution of 1:50 (under 40x lens).