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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 20284-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RADIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IVLPSDGFQ VDLEANCLDD SIYQHLLYVR HFLWGLRSRA SPGSPGRPGS GASQPVCPEG MHHVVLDGHL EAPSCPLAPR DPGPAAREVA PERTLPLRGA PWAQAPPGRQ PGRGGSQAGP PHTDSSCLLT PPSTPLGPEP GDPDWPESGG PCGKALPERQ RNGPSGLRGA APEGDSAALA EESPPAPSSR SSSTEDFCYV FTVELERGPS GLGMGLIDGM HTHLGAPGLY IQTLLPGSPA AADGRLSLGD RILEVNGSSL LGLGYLRAVD LIRHGGKKMR FLVAKSDVET AKKIHFRTPP L (776-1075 aa encoded by BC004919)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using RADIL antibody 20284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RADIL (IP:20284-1-AP, 5ug; Detection:20284-1-AP 1:800) with HepG2 cells lysate 1720ug.
