600-401-432

antibody from Rockland Immunochemicals, Inc.

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-432

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-432, RRID:AB_2201907

Product name

Anti-TGF beta 1 (RABBIT) Antibody - 600-401-432

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Sepsis-induced lung inflammation is modulated by insulin.

Filgueiras LR, Capelozzi VL, Martins JO, Jancar S
BMC pulmonary medicine 2014 Nov 15;14:177

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis using Rockland's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) (611-132-122) diluted 1:2,500. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot