Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12216-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12216-1-AP, RRID:AB_2086929
- Product name
- Cathepsin B antibody
- Antibody type
- Polyclonal
- Description
- Cathepsin B antibody (Cat. #12216-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells.
Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.
1,3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice.
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
MEHP induces pyroptosis and autophagy alternation by cathepsin B activation in INS-1 cells.
Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.
A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Cilostazol alleviate nicotine induced cardiomyocytes hypertrophy through modulation of autophagy by CTSB/ROS/p38MAPK/JNK feedback loop.
Nicotine induces cardiac toxicity through blocking mitophagic clearance in young adult rat.
Heat shock factor 4 regulates lysosome activity by modulating the αB-crystallin-ATP6V1A-mTOR complex in ocular lens.
Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.
GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.
Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial-lysosomal crosstalk.
Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.
Dietary eicosapentaenoic acid normalizes hippocampal omega-3 and 6 polyunsaturated fatty acid profile, attenuates glial activation and regulates BDNF function in a rodent model of neuroinflammation induced by central interleukin-1β administration.
NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.
A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.
Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.
The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.
Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.
Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.
Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.
Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.
Peng S, Yang Q, Li H, Pan Y, Wang J, Hu P, Zhang N
International journal of medical sciences 2021;18(6):1484-1491
International journal of medical sciences 2021;18(6):1484-1491
Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.
Zuo SQ, Li C, Liu YL, Tan YH, Wan X, Xu T, Li Q, Wang L, Wu YL, Deng FM, Tang B
FEBS open bio 2021 Oct;11(10):2705-2714
FEBS open bio 2021 Oct;11(10):2705-2714
1,3-dichloro-2-propanol induced hepatic lipid accumulation by inhibiting autophagy via AKT/mTOR/FOXO1 pathway in mice.
Fan Y, Lu J, Liu J, Zhang R, Yu Z, Guan S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112578
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2021 Nov;157:112578
Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.
Yuan L, Chu B, Chen S, Li Y, Liu N, Zhu Y, Zhou D
Microorganisms 2021 Nov 16;9(11)
Microorganisms 2021 Nov 16;9(11)
MEHP induces pyroptosis and autophagy alternation by cathepsin B activation in INS-1 cells.
Jiang L, Qiu T, Yao X, Chen H, Yao K, Sun X, Yang G, Jiang L, Zhang C, Wang N, Zhang H, Liu X
Environmental science and pollution research international 2021 Dec;28(47):66628-66642
Environmental science and pollution research international 2021 Dec;28(47):66628-66642
Curcumin analogue AI-44 alleviates MSU-induced gouty arthritis in mice via inhibiting cathepsin B-mediated NLRP3 inflammasome activation.
Gu Y, Zhu Y, Deng G, Liu S, Sun Y, Lv W
International immunopharmacology 2021 Apr;93:107375
International immunopharmacology 2021 Apr;93:107375
A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.
Chi H, Hao W, Qi X, Zhang T, Dong Y, Gao H, Wei C, Shi W
Experimental eye research 2021 Apr;205:108509
Experimental eye research 2021 Apr;205:108509
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Hu J, Ding X, Tian S, Chu Y, Liu Z, Li Y, Li X, Wang G, Wang L, Wang Z
Cell death & disease 2021 Apr 12;12(4):391
Cell death & disease 2021 Apr 12;12(4):391
Cilostazol alleviate nicotine induced cardiomyocytes hypertrophy through modulation of autophagy by CTSB/ROS/p38MAPK/JNK feedback loop.
Wang SY, Ni X, Hu KQ, Meng FL, Li M, Ma XL, Meng TT, Wu HH, Ge D, Zhao J, Li Y, Su GH
International journal of biological sciences 2020;16(11):2001-2013
International journal of biological sciences 2020;16(11):2001-2013
Nicotine induces cardiac toxicity through blocking mitophagic clearance in young adult rat.
Jia G, Meng Z, Liu C, Ma X, Gao J, Liu J, Guo R, Yan Z, Christopher T, Lopez B, Liu W, Dai H, Lau WB, Jiao X, Zhao J, Wang ZX, Cao J, Wang Y
Life sciences 2020 Sep 15;257:118084
Life sciences 2020 Sep 15;257:118084
Heat shock factor 4 regulates lysosome activity by modulating the αB-crystallin-ATP6V1A-mTOR complex in ocular lens.
Cui X, Feng R, Wang J, Du C, Pi X, Chen D, Li J, Li H, Zhang J, Zhang J, Mu H, Zhang F, Liu M, Hu Y
Biochimica et biophysica acta. General subjects 2020 Mar;1864(3):129496
Biochimica et biophysica acta. General subjects 2020 Mar;1864(3):129496
Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.
Zhang J, Velmeshev D, Hashimoto K, Huang YH, Hofmann JW, Shi X, Chen J, Leidal AM, Dishart JG, Cahill MK, Kelley KW, Liddelow SA, Seeley WW, Miller BL, Walther TC, Farese RV Jr, Taylor JP, Ullian EM, Huang B, Debnath J, Wittmann T, Kriegstein AR, Huang EJ
Nature 2020 Dec;588(7838):459-465
Nature 2020 Dec;588(7838):459-465
GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.
Chen D, Hou Z, Jiang D, Zheng M, Li G, Zhang Y, Li R, Lin H, Chang J, Zeng H, Guo JT, Zhao X
Emerging microbes & infections 2019;8(1):1511-1523
Emerging microbes & infections 2019;8(1):1511-1523
Inhibition of PI3K/mTOR increased the sensitivity of hepatocellular carcinoma cells to cisplatin via interference with mitochondrial-lysosomal crosstalk.
Sheng J, Shen L, Sun L, Zhang X, Cui R, Wang L
Cell proliferation 2019 May;52(3):e12609
Cell proliferation 2019 May;52(3):e12609
Alantolactone induces apoptosis and improves chemosensitivity of pancreatic cancer cells by impairment of autophagy-lysosome pathway via targeting TFEB.
He R, Shi X, Zhou M, Zhao Y, Pan S, Zhao C, Guo X, Wang M, Li X, Qin R
Toxicology and applied pharmacology 2018 Oct 1;356:159-171
Toxicology and applied pharmacology 2018 Oct 1;356:159-171
Dietary eicosapentaenoic acid normalizes hippocampal omega-3 and 6 polyunsaturated fatty acid profile, attenuates glial activation and regulates BDNF function in a rodent model of neuroinflammation induced by central interleukin-1β administration.
Dong Y, Xu M, Kalueff AV, Song C
European journal of nutrition 2018 Aug;57(5):1781-1791
European journal of nutrition 2018 Aug;57(5):1781-1791
NDRG1 inhibition sensitizes osteosarcoma cells to combretastatin A-4 through targeting autophagy.
Wang H, Li W, Xu J, Zhang T, Zuo D, Zhou Z, Lin B, Wang G, Wang Z, Sun W, Sun M, Chang S, Cai Z, Hua Y
Cell death & disease 2017 Sep 14;8(9):e3048
Cell death & disease 2017 Sep 14;8(9):e3048
A novel acridine derivative, LS-1-10 inhibits autophagic degradation and triggers apoptosis in colon cancer cells.
Fu W, Li X, Lu X, Zhang L, Li R, Zhang N, Liu S, Yang X, Wang Y, Zhao Y, Meng X, Zhu WG
Cell death & disease 2017 Oct 5;8(10):e3086
Cell death & disease 2017 Oct 5;8(10):e3086
Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.
Wang J, Wu Y, Guo J, Fei X, Yu L, Ma S
Oncotarget 2017 Oct 10;8(47):81880-81891
Oncotarget 2017 Oct 10;8(47):81880-81891
The role of NLRP3-CASP1 in inflammasome-mediated neuroinflammation and autophagy dysfunction in manganese-induced, hippocampal-dependent impairment of learning and memory ability.
Wang D, Zhang J, Jiang W, Cao Z, Zhao F, Cai T, Aschner M, Luo W
Autophagy 2017 May 4;13(5):914-927
Autophagy 2017 May 4;13(5):914-927
Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux.
Xie X, Yi W, Zhang P, Wu N, Yan Q, Yang H, Tian C, Xiang S, Du M, Getachew Assefa E, Zuo X, Ying C
Nutrients 2017 May 14;9(5)
Nutrients 2017 May 14;9(5)
Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients.
Sun T, Jiang D, Zhang L, Su Q, Mao W, Jiang C
Oncology letters 2016 Jan;11(1):575-583
Oncology letters 2016 Jan;11(1):575-583
Expression of cystatin C and its effect on EC9706 cells in esophageal carcinoma.
Yan Y, Zhou K, Wang L, Zhou Y, Chen X, Fan Q
International journal of clinical and experimental pathology 2015;8(9):10102-11
International journal of clinical and experimental pathology 2015;8(9):10102-11
Host-derived, pore-forming toxin-like protein and trefoil factor complex protects the host against microbial infection.
Xiang Y, Yan C, Guo X, Zhou K, Li S, Gao Q, Wang X, Zhao F, Liu J, Lee WH, Zhang Y
Proceedings of the National Academy of Sciences of the United States of America 2014 May 6;111(18):6702-7
Proceedings of the National Academy of Sciences of the United States of America 2014 May 6;111(18):6702-7
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12216-1-AP(CTSB antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 12216-1-AP(CTSB antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Cathepsin B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Cathepsin B. This antibody recognizes human, mouse antigen. The Cathepsin B antibody has been validated for the following applications: ELISA, WB, IHC analysis.