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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-35937 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOL8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa nuclear.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NOL8 Polyclonal Antibody detects NOL8 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5 % SDS-PAGE, and blotted with NOL8 Polyclonal Antibody (Product # PA5-35937) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NOL8 was performed in HeLa whole cell extracts using 5 µg of NOL8 Polyclonal Antibody (Product # PA5-35937). Samples were transferred to a membrane and probed with NOL8 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
