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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- 34-3500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ephrin B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.
Expression profiling by high-throughput immunohistochemistry.
Hogerheyde TA, Stephenson SA, Harkin DG, Bray LJ, Madden PW, Woolf MI, Richardson NA
Experimental eye research 2013 Feb;107:110-20
Experimental eye research 2013 Feb;107:110-20
Expression profiling by high-throughput immunohistochemistry.
Warford A, Howat W, McCafferty J
Journal of immunological methods 2004 Jul;290(1-2):81-92
Journal of immunological methods 2004 Jul;290(1-2):81-92
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed using membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and NTERA-2 (Lane 2). The blots were probed with Anti-Ephrin-B1 Rabbit Polyclonal Antibody (Product # 34-3500, 2-4 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 38 kDa band corresponding to Ephrin-B1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
