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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB4731 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Ephrin-B1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Ephrin-B1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Ephrin-A1, -A2, -A4, -B2, recombinant human Ephrin-A3, -A4, -A5, or -B3 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
AAA53231- Isotype
- IgG
- Antibody clone number
- 94038
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
Jin W, Qi S, Luo H
BMC immunology 2011 Dec 19;12:68
BMC immunology 2011 Dec 19;12:68
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse Ephrin-B1 by Western Blot. Western blot shows lysates of mouse lung tissue and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Ephrin-B1 Monoclonal Antibody (Catalog # MAB4731) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Ephrin-B1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
