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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB1408 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cystatin B/Stefin B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Cystatin B in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cystatins A and S, recombinant mouse Cystatin B, rhFetuin A, and rhHPRG (Histidine-Proline-Rich Glycoprotein) is observed and approximately 5% to 10% cross-reactivity with rhCystatins C, D, E/M, SA, SN, and rhFetuin B is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dysregulation of macrophage-secreted cathepsin B contributes to HIV-1-linked neuronal apoptosis.
Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression.
Rodriguez-Franco EJ, Cantres-Rosario YM, Plaud-Valentin M, Romeu R, RodrĂguez Y, Skolasky R, MelĂ©ndez V, Cadilla CL, Melendez LM
PloS one 2012;7(5):e36571
PloS one 2012;7(5):e36571
Cystatin B as a tissue and urinary biomarker of bladder cancer recurrence and disease progression.
Feldman AS, Banyard J, Wu CL, McDougal WS, Zetter BR
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 1;15(3):1024-31
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 1;15(3):1024-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Cystatin B by Western Blot. Detection of Human Cystatin B by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neutralization of Cystatin B Activity by Human Cystatin B Antibody. Neutralization of Cystatin B Activity by Human Cystatin B Antibody.
