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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ4523 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Stefin B Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2B6
- Vial size
- 100 µg
- Storage
- After reconstitution, the Stefin B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20deg;C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U-87 MG, 2) SW620, 3) U-937 and 4) Caco-2 cell lysate with Stefin B antibody at 0.5ug/ml. Predicted molecular weight ~11 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with Stefin B antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human PC3 cells with Stefin B antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Stefin B antibody.
