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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-117896 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cystatin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cystatin B Polyclonal Antibody (Product # PA5-117896) at 1:500 dilution. Lane A: U87-MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Cystatin B in human esophagus with Cystatin B Polyclonal Antibody (Product # PA5-117896, 1:5,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Cystatin B in human urinary bladder with Cystatin B Polyclonal Antibody (Product # PA5-117896, 1:1,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cystatin B Immunoprecipitation using: Lane A: 0.5 mg U87-MG Whole Cell Lysate 4 µL with Cystatin B Polyclonal Antibody (Product # PA5-117896) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Cystatin B Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa.
