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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AB0016-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0016-200, RRID:AB_2333133
- Product name
- Anti-CX43
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 800 µg
- Concentration
- 4 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin.
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Martins-Marques T, Pinho MJ, Zuzarte M, Oliveira C, Pereira P, Sluijter JP, Gomes C, Girao H
Journal of extracellular vesicles 2016;5:32538
Journal of extracellular vesicles 2016;5:32538
Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart.
Martins-Marques T, Anjo SI, Pereira P, Manadas B, Girão H
Molecular & cellular proteomics : MCP 2015 Nov;14(11):3040-55
Molecular & cellular proteomics : MCP 2015 Nov;14(11):3040-55
AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions.
Ribeiro-Rodrigues TM, Catarino S, Marques C, Ferreira JV, Martins-Marques T, Pereira P, Girão H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4629-41
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cell lysates at 100 µg per lane
- Sample type
- COS-7 cell line
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with methanol
- Sample type
- primary RPE cells
- Primary Ab dilution
- 1/100
- Conjugate
- Unconjugated
